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New Full Length 16s, 18s, and ITS1-4 Sequencing!!!

Now Available 600bp-1500bp Amplicon Sequencing for Improved Diversity Studies

Our Sequencing Platforms

MRDNA MiSeq 16s Sequencing

Illumina NovaSeq

 

The NovaSeq 6000 System is the latest high throughput sequencing instrument released by Illumina. With up to 6Tb of data and 20 Billion paired-end reads made possible by the Illumina NovaSeq...

Illumina MiSeq

 

Illumina’s MiSeq is quickly becoming the sequencer of choice for researchers, such as yourself, to carryout their genomic and targeted resequencing studies. The highly experienced team at MR DNA is…

 

 

 

Pacbio Sequel

 

The PacBio Sequel is the newest sequencing platform released by Pacific Biosciences. The PacBio Sequel, aptly named, follows the release of the PacBio RS II System; one of the first to offer read lengths greater than 20Kb, and the PacBio Sequel is no different...

MRDNA HiSeq 16s Sequencing

Illumina HiSeq

 

  The HiSeq has been discontinued and all applications moved to the NovaSeq

 

 

 

SeqStudio

 

Most Sanger methods are now on Pac Bio High Fidelity Long read systems.  We feel data is much better and although cost slightly higher it is worth it for most applications. 

 

 

16s rRNA Sequencing &

Amplicon sequencing

 

The 16s rRNA gene for bacteria and archaea, the ITS regions for fungi, the 18s regions for general eukaryote, coi sequencing etc. are the ideal target to complete microbiome studies. MR DNA has extensive arrays of different ribosomal, phylogenetic markers and functional assays in-house

DNA and RNA Sequencing

 

We offer a wide range of NGS platforms, making whole genome sequencing all the more affordable. From small microbial genomes to larger eukaryote genomes, whole genome, RNAseq, transcriptome, isoseq, resequencing, metagenome, metatranscriptome, bisulfite, exome sequencing, target enrichment, reduced representation are just examples of our broad range of services.

Microbiome Sequencing

 

MRDNA is passionate about microbiome research. In addition to our 16s sequencing services, ITS sequencing, 18s, COI, rpoB, functional genes or any other type of diversity assay you can imagine or create.  we can help you generate the data you need.  small project or large project we are your full service end to end solution.

Our Services

Today's Research

Syntroph diversity and abundance in anaerobic digestion revealed through a comparative core microbiome approach

 

Anaerobic digestion is an important biotechnology treatment process for conversion of waste to energy. In this study, a comparative core microbiome approach, i.e., determining taxa that are shared in functioning digesters but not shared in non-functioning digesters, was used to determine microbial taxa that could play key roles for effective anaerobic digestion. Anaerobic digester functions were impaired by adding the broad-spectrum antimicrobial triclosan (TCS) or triclocarban (TCC) at different concentrations, and the core microbiomes in both functioning and non-functioning anaerobic digesters were compared. Digesters treated with high (2500 mg/kg) or medium (450 mg/kg) TCS and high (850 mg/kg) TCC concentrations lost their function, i.e., methane production decreased, effluent volatile fatty acid concentrations increased, and pH decreased. Changes in microbial community diversity and compositions were assessed using 16S rRNA gene amplicon sequencing. Microbial richness decreased significantly in non-functioning digesters (p < 0.001). Microbial community compositions in non-functioning digesters significantly differed from those in functioning digesters (p = 0.001, ANOSIM). Microbes identified as potentially key taxa included previously known fatty acid-degrading syntrophs and amino acid-degrading syntrophs. A diverse group of syntrophs detected in this study had low relative abundance in functioning digesters, suggesting the importance of rare microbes in anaerobic digester operation. The comparative microbiome approach used in this study can be applied to other microbial systems where a community-driven biological phenomena can be observed directly.

 

 

Fujimoto, M., Carey, D. E., Zitomer, D. H., & McNamara, P. J. (2019). Syntroph diversity and abundance in anaerobic digestion revealed through a comparative core microbiome approach. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-019-09862-4

In The News

In The News

16s Sequencing

16s sequencing is one of the go to sequencing methods to complete microbiome studies. 16s sequencing involves sequencing of the 16s rRNA gene found in all bacteria and archaea. The fact that the 16s rRNA gene can be found in all prokaryotes make the 16s gene the ideal candidate to characterize the microbiome of numerous environments. Another key factor that makes the 16s gene an ideal candidate for microbiome characterization is the fact that there are both highly conserved regions and highly variable regions within the gene. The highly conserved regions within the 16s rRNA gene make great targets for PCR primers bind to and replicate thereby producing millions of copies of the targeted hypervariable region that is flanked by the highly conserved regions. The 16s rRNA gene contains 9 hypervariable regions that can be targeted in order to gain insight into the diversity amongst prokaryotes.

 

16s rRNA sequencing has grown in popularity over the last decade due in large part to next-generation sequencing. NGS, also known as high-throughput sequencing, has drastically reduced the cost of DNA sequencing due to its capability to sequence hundreds of samples at a time. In the past, capillary electrophoresis was able to produce ~1Mb per run, and today, with instruments such as the Illumina HiSeq, we are able to produce ~500 Gb per run. Until recently, one metric that Sanger sequencing had as an advantage over NGS instruments such as the Illumina MiSeq and Ion Torrent was read length. On average, capillary electrophoresis was able to achieve read lengths up to 1,000bp in comparison to the 400-600bp read lengths provided by Illumina and Ion Torrent. However, the PacBio Sequel now allows researchers the ability to achieve read lengths ranging from 10-30Kb. What does this mean for 16s rRNA sequencing? We can now sequence the entire 16s rRNA gene, which is approximately 1.5Kb in length. Whether your goal is to target one specific hypervarible region or the entire 16s rRNA gene, there is a NGS platform for you.

 

 

Metagenome Sequencing

Metagenome sequencing is a term that can cause a lot of confusion, as well as bring so much joy to microbiologists around the world.  Metagenome sequencing is a sequencing method that investigates the DNA extracted from an environmental sample as a whole. Whereas certain DNA sequencing techniques will target a specific organism from a specific environment, metagenome sequencing targets all microbial organisms found in a certain environment. There are predominantly two methods used to completely metagenomics studies, 16s rRNA sequencing and Shotgun metagenome sequencing. The difference between the two sequencing methods can be found in their names. 16s rRNA sequencing, as you would expect, targets only the 16s rRNA gene, while Shotgun metagenome sequencing targets all genes present in your sample. How is this accomplished?

 

Shotgun metagenome sequencing involves the random shearing of all DNA present in a particular sample. These smaller fragments are sequenced on NGS platforms such the Illumina MiSeq and then reassembled. Because shotgun metagenomics is non-discriminatory, not only are you able to gain taxonomic information, similar to 16s sequencing, but you are also able to gain insight to the presence of functional genes as well. Overall, shotgun metagenome sequencing provides a more complete picture of your environmental sample. NGS platforms such as the Illumina HiSeq allow researchers to ability to sequence their metagenome samples to a much greater depth. Why is sequencing depth important? Naturally, the most abundant organisms in your sample will receive the most amounts of data, and because you are not specifically targeting certain organisms, without sufficient sequencing coverage, you run the risk of not identifying those organisms that may be underrepresented. From the human microbiome, to pond water, and to the rumen of cattle, shotgun metagenome sequencing paired with NGS platforms has the ability to take your ecological study to the next level.

 

 

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BIOINFORMATICS SERVICES

 

Over 15 years of applied bioinformatics experience. Do not waste your time and money we can help you with any type of project. From full support to just helping you…

 

Programs

Molecular CONSULTING

 

We offer consulting for almost any type of molecular or bioinformatic needs. Whether you just want to know how many biological replications you might need for your 454 microbial diversity…

 

 

MICROBIOME RESEARCH

 

16S rRNA pyrosequencing service, microbiome, metagenome, transcriptomes, genomes, bioinformatics, small RNA, Illumina, Ion Torrent, 454 pyrosequencing service. pricing for sequencing services. Inexpensive methods to evaluate microbial diversity using barcoded approach…

 

 

 

MOLECULAR METHOD DEVELOPMENT

 

We have 20 years of continuous experience developing novel molecular assays, bioinformatics tools. We are currently developing next next generation assays in collaboration with our partners.

 

 

 

 

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